introduction: the purpose of this study was to compare the inhibitory effect of heat-killed candida albicans and dentin powder on the antibacterial activity of chlorhexidine (chx) against enterococcus faecalis and streptococcus sanguis . materials and methods: the antibacterial effect of each group was determined by measuring the zone of inhibition diameter in millimeters after incubation at 37...

Membrane currents evoked by repeated noxious heat stimuli (43-47 degrees C) of 3 s duration were investigated in acutely dissociated dorsal root ganglion (DRG) neurones of adult rats. The heat stimuli generated by a fast solution exchanger had a rise time of 114 +/- 6 ms and a fall time of 146 +/- 13 ms. When heat stimuli were applied to heat-sensitive small (< or = 32.5 microm) DRG neurones, a...

lysozyme is considered as part of the innate immune system. it has stimulated considerable interest as a natural food preservative. lysozyme has been shown to be effective in preserving a variety of foods such as fresh fruits and vegetables, meats, seafood and wine, for which many japanese patents have been granted. the relatively high thermal stability of lysozyme also makes it attractive for ...

Three methods for quantifying skeletal alkaline phosphatase (ALP; EC 3.1.3.1) activity/immunoactivity in serum--heat inactivation, wheat germ agglutinin (WGA) precipitation, and an immunoradiometric assay--were tested for recovery and specificity and applied to 81 sera collected from 14 postmenopausal osteoporotic subjects. The heat-inactivation and WGA precipitation assays showed relative reco...

The causal infectious agents of TSEs (transmissible spongiform encephalopathies or prion diseases) are renowned for their resistance to complete inactivation. Survival of TSE infectivity after autoclaving potentially compromises many procedures where TSE infectivity may be present, including surgical instrument sterilization. In the present study, the heat inactivation properties of five differ...

protein aggregation is a problem in biotechnology. high temperature is one of the most important reasons to enhance enzyme inactivation and aggregation in industrial systems. this work focuses on the effect of tio2 and sio2 nanoparticles on refolding and reactivation of lysozyme. in the presence of tio2 and sio2 nanoparticles, after enzyme heat treatment at 98◦c for 30 min, not only aggregates ...

The performance of two immunoturbidimetric modifications for rheumatoid factor (RF) testing, which differ with respect to the means of complement inactivation (heat treatment and inactivation with polyvinyl sulphonate), were compared in serum samples from 87 patients with rheumatoid arthritis (RA) and from 403 healthy subjects. IgM-rheumatoid factor titres were also measured with an enzyme link...

background: bone-specific alkaline phosphatase (bap) is synthesized by the osteoblasts and is presumed to be involved in the calcification of bone matrix, though its precise role in the formation process is unknown. the aim of the present study was to measure the bap activity in paget&apos;s and normal specimens by two different techniques. methods: total alp (tap) as well as bap activity-measu...